Long-read and RNA

Date 11 - 12 March 2021
Speaker(s) Geert van Geest
ECTS 0.5
Fees *academic: 120 CHF   -   for-profit: 600 CHF
Cancellation deadline 25 Feb 2021
City Bern
*academic fee includes non for-profit organisations as well as unemployed participants at the time of application.

Overview

For the last few years, research in (c)DNA has been dominated by short-read sequencing. Although this has revolutionized the way we answer biological questions, it has its limitations. One important limitation is read length. The invention of high-throughput long-read sequencing has enabled us to solve questions on genome assembly, haplotyping, structural variation and alternative splicing. In this course, the participant will get acquainted with the major long-read sequencing technologies (PacBio SMRT and Oxford Nanopore Technology), and get experience with hands-on work involving long-read data.

Audience

This course is addressed to people that are working with or will be working with long-read (RNA-seq) data.

Learning outcomes

At the end of the course, the participants are expected to:

  • Understand the basics behind PacBio SMRT sequencing and Oxford Nanopore Technology sequencing
  • Use the command line to perform quality control and read alignment of long-read sequencing data
  • Do a differential isoform expression analysis

Prerequisites

Knowledge / competencies

This course is designed for anyone interested to work with long-read (RNA-seq) data and there is a requirement for basic UNIX knowledge.

Technical

You are required to bring your own laptop. Software installation instructions will be communicated before the course.

Schedule - CET time zone

Application

The registration fees for academics are 120 CHF and 600 CHF for for-profit companies.

You will be informed by email of your registration confirmation. Upon reception of the confirmation email, participants will be asked to confirm attendance by paying the fees within 5 days.

Deadline for free-of-charge cancellation is set to 25/02/2021. Cancellation after this date will not be reimbursed. Please note that participation in SIB courses is subject to our general conditions.

Venue and Time

Universität Bern, room 331 / 3. OG West, Hochschulstrasse 4.

The course will start at 9:00 and end around 17:00. Precise information will be provided to the participants in due time.

Additional information

Coordination: Valeria Di Cola

We will recommend 0.5 ECTS credits for this course (given a passed exam at the end of the course).

You are welcome to register to the SIB courses mailing list to be informed of all future courses and workshops, as well as all important deadlines using the form here.

Please note that participation in SIB courses is subject to our general conditions.

SIB abides by the ELIXIR Code of Conduct. Participants of SIB courses are also required to abide by the same code.

For more information, please contact training@sib.swiss. -->